<Slide 1-3>
Check PCR products with primers(Forward, Reverse）at 56 degree.
As template DNA, we used reverse transcribed cDNA from "Firstchoice human brain reference total RNA (Ambion, cat. no. 6051)".

Slide 1: PCR amplification results 15 genes including GAPDH
Slide 2: PCR amplification results 15 genes including SKIL
Slide 3: PCR amplification results 10 genes including MEIS3

<Slide 4>
Check PCR products at 58 degree for 8 genes, which are bad products from Slide 1-3.

<Slide 5>
Check PCR products at 60 degree for 3 genes, which are bad products from Slide 4.

<Slide 6-7>
As a result from Slide 5, we reordered "LOX2" and "CCAR2" new primers.
Check PCR products using new Forward primer and new Reverse primers.
Slide 6: condition at 58 degree
Slide 7: condition at 60 degree
Finally we determined new primer sets at 60 degree for 2 genes.

<Slide 8>
Reference；DNA ladder marker